Premium
Temporal changes in microvessel leakiness during wound healing discriminated by in vivo fluorescence recovery after photobleaching
Author(s) -
Machado Maria J. C.,
Mitchell Christopher A.
Publication year - 2011
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2011.208355
Subject(s) - fluorescence recovery after photobleaching , in vivo , wound healing , microvessel , endothelium , anatomy , dextran , chemistry , plexus , blood vessel , pathology , biology , medicine , endocrinology , immunohistochemistry , immunology , biochemistry , microbiology and biotechnology , membrane
Non‐Technical Summary Wound closure depends on vascular ingrowth into the injured area and this process has traditionally been studied in tissue sections of animals ex vivo . Following the creation of a wound within a surgically implanted dorsal window chamber, we captured microscopic images of the ingrowing vasculature on successive days after injury. Using a combination of surgical, confocal microscopic and mathematical techniques we quantified the flux of plasma into and around vessels including: newly formed vessel sprouts, nascent flowing vascular segments and pre‐existing vessels within the same wound. From these analyses we are able to discriminate: (1) vessels with differing maturity, (2) that vascular sprouts get progressively less leaky and (3) TNP‐470 (an anti‐angiogenic agent), reduces leakiness in sprouts with co‐incident secondary effects on pre‐existing vessels. These techniques can be used to assess both functional maturity and the effects of therapeutics on the vasculature of healing wounds.