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Local release of ATP into the arterial inflow and venous drainage of human skeletal muscle: insight from ATP determination with the intravascular microdialysis technique
Author(s) -
Mortensen Stefan P.,
Thaning Pia,
Nyberg Michael,
Saltin Bengt,
Hellsten Ylva
Publication year - 2011
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2010.203034
Subject(s) - microdialysis , skeletal muscle , cardiology , medicine , inflow , chemistry , mechanics , central nervous system , physics
Non‐technical summary ATP has been proposed to contribute to the local regulation of skeletal muscle blood flow by inducing local vasodilatation. ATP is continuously released, degraded and taken up by cells and the physiological levels are therefore difficult to determine. In the present investigation, we used a novel technique involving microdialysis probes to determine plasma ATP levels in blood vessels supplying and draining resting and contracting human skeletal muscle and to investigate the stimuli for ATP release. The results show a local release of ATP into arterial and venous blood. In addition, we found that thigh compression is a stimulus for ATP release. Furthermore, we find that the half‐life of ATP in arterial blood is <1 s.