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Preferential skeletal muscle myosin loss in response to mechanical silencing in a novel rat intensive care unit model: underlying mechanisms
Author(s) -
Ochala Julien,
Gustafson AnnMarie,
Diez Monica Llano,
Renaud Guillaume,
Li Meishan,
Aare Sudhakar,
Qaisar Rizwan,
Banduseela Varuna C.,
Hedström Yvette,
Tang Xiaorui,
Dworkin Barry,
Ford G. Charles,
Nair K. Sreekumaran,
Perera Sue,
Gautel Mathias,
Larsson Lars
Publication year - 2011
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2010.202044
Subject(s) - skeletal muscle , myosin , chemistry , gene silencing , neuroscience , microbiology and biotechnology , medicine , biology , anatomy , biochemistry , gene
Non‐technical summary Wasting and severely impaired function of skeletal muscle is frequently observed in critically ill intensive care unit (ICU) patients, with negative consequences for recovery and quality of life. An experimental rat ICU model has been used to study the mechanisms underlying this unique wasting condition in neuromuscularly blocked and mechanically ventilated animals at durations varying between 6 h and 2 weeks. The complete ‘mechanical silencing’ of skeletal muscle (removal of both weight bearing and activation) resulted in a specific myopathy frequently observed in ICU patients and characterized by a preferential loss of the motor protein myosin. A highly complex and coordinated protein synthesis and degradation system was observed in the time‐resolved analyses. It is suggested the ‘mechanical silencing’ of skeletal muscle is a dominating factor triggering the specific myopathy associated with the ICU intervention, and strongly supporting the importance of interventions counteracting the complete unloading in ICU patients.