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High‐resolution simultaneous voltage and Ca 2+ imaging
Author(s) -
Vogt Kaspar E.,
Gerharz Stephan,
Graham Jeremy,
Canepari Marco
Publication year - 2011
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2010.200220
Subject(s) - voltage , resolution (logic) , optical imaging , chemistry , materials science , physics , computer science , optics , artificial intelligence , quantum mechanics
Combining voltage and Ca 2+ imaging allows the correlation of electrical and chemical activity at sub‐cellular level. Here we describe a novel apparatus designed to obtain simultaneous voltage and Ca 2+ measurements with single‐trial resolution from sites as small as a few microns. These measurements can be obtained with negligible optical cross‐talk between the two signals and negligible photo‐damage of the preparation. The capability of the technique was assessed recording either from individual neurons in brain slices or from networks of cultured neurons. The present achievements open the gate to many novel physiological investigations requiring simultaneous measurement of voltage and Ca 2+ signals.