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Electrical activity‐triggered glucagon‐like peptide‐1 secretion from primary murine L‐cells
Author(s) -
Rogers G. J.,
Tolhurst G.,
Ramzan A.,
Habib A. M.,
Parker H. E.,
Gribble F. M.,
Reimann F.
Publication year - 2011
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2010.198069
Subject(s) - secretion , enteroendocrine cell , hormone , microbiology and biotechnology , glucagon , glucagon like peptide 1 , transgene , genetically modified mouse , peptide hormone , biology , chemistry , endocrinology , endocrine system , diabetes mellitus , biochemistry , type 2 diabetes , gene
Non‐technical summary Glucagon like peptide 1 (GLP‐1) based therapies are now widely used for the treatment of diabetes. The physiological source of the hormone is the intestinal L‐cell, and attempts to boost secretion have been hindered by difficulties in distinguishing these cells from their epithelial neighbours and our consequent limited understanding of their physiology. Using recently developed transgenic mice with fluorescently labelled L‐cells, we show that these cells are electrically active and use voltage‐gated ion channels to couple the presence of nutrients to the secretion of GLP‐1. We present the identification and characterisation of the ion channels. This improves our understanding of enteroendocrine physiology and will support therapeutic programmes aiming to target gut hormone secretion.