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Ethinyl oestradiol administration in women suppresses synthesis of collagen in tendon in response to exercise
Author(s) -
Hansen Mette,
Koskinen Satu O.,
Petersen Susanne G.,
Doessing Simon,
Frystyk Jan,
Flyvbjerg Allan,
Westh Eva,
Magnusson S. Peter,
Kjaer Michael,
Langberg Henning
Publication year - 2008
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2007.147348
Subject(s) - tendon , medicine , patellar tendon , endocrinology , microdialysis , endogeny , type i collagen , surgery , central nervous system
Women are at greater risk than men of sustaining certain kinds of injury and diseases of collagen‐rich tissues. To determine whether a high level of oestradiol has an acute influence on collagen synthesis in tendons at rest and in response to exercise, one‐legged kicking exercise was performed for 60 min at 67% of maximum power by healthy, young oral contraceptive (OC) users when circulating synthetic (ethinyl) oestradiol was high ( n = 11, HE‐OC) and compared to similar women who had never used OCs when circulating endogenous oestrogen was low ( n = 12, LE‐NOC). Interstitial fluid was collected 24 h post‐exercise through microdialysis catheters placed anterior to the patellar tendon in both legs and subsequently analysed for the amino‐terminal propeptide of type I collagen (PINP), a marker of tendon collagen synthesis. To determine the long‐term effect of OC usage, patellar tendon cross‐sectional area (CSA) was measured by magnetic resonance imaging (MRI). A lower exercise‐induced increase in tendon collagen synthesis was observed in HE‐OC than in LE‐NOC (ΔPINP (mean ± s.e.m. ) 1.5 ± 5.3 versus 24.2 ± 9.4 ng ml −1 , P < 0.05). Furthermore, serum and the interstitial peritendinous tissue concentrations of insulin‐like growth factor I (IGF‐I) and IGF‐binding proteins showed a reduced bioavailability in HE‐OC compared with results in LE‐NOC. No difference in patellar tendon CSA was observed between groups. In conclusion, the selective increase in tendon collagen synthesis in LE‐NOC but not HE‐OC 24 h post‐exercise is consistent with the hypothesis that oestradiol inhibits exercise‐induced collagen synthesis in human tendon. The mechanism behind this is either a direct effect of oestradiol, or an indirect effect via a reduction in levels of free IGF‐I. However, the data did not indicate any long‐term effect on tendon size associated with chronic OC use.