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Stimulated exocytosis of endosomes in goldfish retinal bipolar neurons
Author(s) -
Coggins Michael R.,
Grabner Chad P.,
Almers Wolfhard,
Zenisek David
Publication year - 2007
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2007.140848
Subject(s) - exocytosis , endocytosis , ribbon synapse , endosome , synaptic vesicle , bulk endocytosis , microbiology and biotechnology , biology , chemistry , vesicle , endocytic cycle , membrane , receptor , biochemistry , intracellular
After exocytosis, synaptic vesicle components are selectively retrieved by clathrin‐mediated endocytosis and then re‐used in future rounds of transmitter release. Under some conditions, synaptic terminals in addition perform bulk endocytosis of large membranous sacs. Bulk endocytosis is less selective than clathrin‐mediated endocytosis and probably internalizes components normally targeted to the plasma membrane. Nonetheless, this process plays a major role in some tonic ribbon‐type synapses, which release neurotransmitter for prolonged periods of time. We show here, that large endosomes formed after strong and prolonged stimulation undergo stimulated exocytosis in retinal bipolar neurons. The result suggests how cells might return erroneously internalized components to the plasma membrane, and also demonstrates that synaptic vesicles are not the only neuronal organelle that stains with styryl dyes and undergoes stimulated exocytosis.