Premium
Developmental changes in brain‐derived neurotrophic factor‐mediated modulations of synaptic activities in the pontine Kölliker–Fuse nucleus of the rat
Author(s) -
Kron Miriam,
Mörschel Michael,
Reuter Julia,
Zhang Weiqi,
Dutschmann Mathias
Publication year - 2007
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2007.134726
Subject(s) - excitatory postsynaptic potential , neuroscience , postsynaptic current , inhibitory postsynaptic potential , brain derived neurotrophic factor , neurotrophic factors , brainstem , postsynaptic potential , slice preparation , neurotransmission , synapse , microinjection , biology , chemistry , electrophysiology , medicine , endocrinology , receptor
The Kölliker–Fuse nucleus (KF), part of the respiratory network, is involved in the modulation of respiratory phase durations in response to peripheral and central afferent inputs. The KF is immature at birth. Developmental changes in its physiological and anatomical properties have yet to be investigated. Since brain‐derived neurotrophic factor (BDNF) is of major importance for the maturation of neuronal networks, we investigated its effects on developmental changes in the KF on different postnatal days (neonatal, P1–5; intermediate, P6–13; juvenile, P14–21) by analysing single neurones in the in vitro slice preparation and network activities in the perfused brainstem preparation in situ . The BDNF had only weak effects on the frequency of mixed excitatory and inhibitory spontaneous postsynaptic currents (sPSCs) in neonatal slice preparations. Postnatally, in the intermediate and juvenile age groups, a significant augmentation of the sPSC frequency was observed in the presence of 100 p m BDNF (+23.5 ± 12.6 and +76.7 ± 28.4%, respectively). Subsequent analyses of BDNF effects on evoked excitatory postsynaptic currents (eEPSCs) revealed significant enhancement of eEPSC amplitude of +20.8 ± 7.0% only in juvenile stages (intermediates, −13.2 ± 4.8%). On the network level, significant modulation of phrenic nerve activity following BDNF microinjection into the KF was also observed only in juveniles. The data suggest that KF neurones are subject to BDNF‐mediated fast synaptic modulation after completion of postnatal maturation. After maturation, BDNF contributes to modulation of fast excitatory neurotransmission in respiratory‐related KF neurones. This may be important for network plasticity associated with the processing of afferent information.