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TrkB is necessary for pruning at the climbing fibre–Purkinje cell synapse in the developing murine cerebellum
Author(s) -
Johnson Erin M.,
Craig Ethan T.,
Yeh Hermes H.
Publication year - 2007
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2007.133561
Subject(s) - synapse , cerebellum , tropomyosin receptor kinase b , neuroscience , purkinje cell , soma , biology , climbing , neurotrophin , dendrite (mathematics) , climbing fiber , microbiology and biotechnology , neurotrophic factors , receptor , genetics , ecology , geometry , mathematics
TrkB, the cognate receptor for brain‐derived neurotrophic factor and neurotrophin‐4, has been implicated in regulating synapse formation in the central nervous system. Here we asked whether TrkB plays a role in the maturation of the climbing fibre–Purkinje cell (CF–PC) synapse. In rodent cerebellum, Purkinje cells are initially innervated by multiple climbing fibres that are subsequently culled to assume the mature mono‐innervated state, and whose contacts translocate from the soma to the dendrites. By employing transgenic mice hypomorphic or null for TrkB expression, our results indicated that perturbation of TrkB in the immature cerebellum resulted in ataxia, that Purkinje cells remained multiply innervated by climbing fibres beyond the normal developmental time frame, and that synaptic transmission at the parallel fibre–Purkinje cell synapse remained functionally unaltered. Mechanistically, we present evidence that attributes the persistence of multiple climbing fibre innervation to an obscured discrimination of relative strengths among competing climbing fibres. Soma‐to‐dendrite translocation of climbing fibre terminals was unaffected. Thus, TrkB regulates pruning but not translocation of nascent CF–PC synaptic contacts.