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Simultaneous inhibition of caudal medullary raphe and retrotrapezoid nucleus decreases breathing and the CO 2 response in conscious rats
Author(s) -
Li Aihua,
Zhou Shawn,
Nattie Eugene
Publication year - 2006
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2006.114504
Subject(s) - raphe , raphe nuclei , serotonergic , central chemoreceptors , agonist , chemistry , medulla , wakefulness , chemoreceptor , anesthesia , endocrinology , neuroscience , medicine , biology , serotonin , receptor , electroencephalography
The medullary raphe (MR) and the retrotrapezoid nucleus (RTN) in the ventral medulla are two of many central chemoreceptor sites. We examine their combined function in conscious rats by focal inhibition using microdialysis. Inhibition of RTN neurons with the GABA A receptor agonist muscimol, with simultaneous dialysis of artificial cerebrospinal fluid (ACSF) in or near to the caudal MR, causes hypoventilation (decrease in the ratio of minute ventilation to oxygen consumption, ) and reduces the ventilatory response to 7% CO 2 by 24%. Inhibition of caudal MR serotonergic neurons with the 5‐HT 1A receptor agonist ( R )‐(+)‐8‐hydroxy‐2(di‐ n ‐propylamino)tetralin (8‐OH‐DPAT), with simultaneous dialysis of ACSF in or near to the RTN, causes hypoventilation but has no significant effect on the CO 2 response. Inhibition of both the RTN and the caudal MR simultaneously produces enhanced hypoventilation and a 51% decrease in the CO 2 response. The effects of treatment on the CO 2 response are similar in wakefulness and in non‐rapid eye movement sleep. Comparison of the effect of 8‐OH‐DPAT microdialysed into a more rostral portion of the MR, where the CO 2 response is reduced by 22%, demonstrates heterogeneity within the MR of the function of serotonergic neurons in breathing. We conclude that serotonergic neurons within the caudal MR provide a non‐CO 2 ‐dependent tonic drive to breathe and potentiate the effects of RTN neurons that contribute to a resting chemical ‘drive to breathe’ as well as the response to added CO 2 . These effects of caudal MR serotonergic neurons could be at a chemoreceptor site, e.g. the RTN, or at ‘downstream’ sites involved in rhythm and pattern generation.