Premium
The influence of intracellular lactate and H + on cell volume in amphibian skeletal muscle
Author(s) -
UsherSmith Juliet A.,
Fraser James A.,
Bailey Peter S. J.,
Griffin Julian L.,
Huang Christopher L.H.
Publication year - 2006
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2006.108316
Subject(s) - intracellular , extracellular , intracellular ph , lactic acid , chemistry , biophysics , osmotic concentration , biochemistry , biology , bacteria , genetics
The combined effects of intracellular lactate and proton accumulation on cell volume, V c , were investigated in resting Rana temporaria striated muscle fibres. Intracellular lactate and H + concentrations were simultaneously increased by exposing resting muscle fibres to extracellular solutions that contained 20–80 m m sodium lactate. Cellular H + and lactate entry was confirmed using pH‐sensitive electrodes and 1 H‐NMR, respectively, and effects on V c were measured using confocal microscope xz‐ scanning. Exposure to extracellular lactate up to 80 m m produced significant changes in pH and intracellular lactate (from a pH of 7.24 ± 0.03, n = 8, and 4.65 ± 1.07 m m , n = 6 , respectively, in control fibres, to 6.59 ± 0.03, n = 4, and 26.41 ± 0.92 m m , n = 3 , respectively) that were comparable to those observed following fatiguing stimulation ( 6.30–6.70 and 18.04 ± 1.78 m m , n = 6 , respectively). Yet, the increase in intracellular osmolarity expected from such an increase in intracellular lactate did not significantly alter V c . Simulation of these experimental results, modified from the charge difference model of Fraser & Huang, demonstrated that such experimental manoeuvres produced changes in intracellular [H + ] and [lactate] comparable to those observed during muscle fatigue, and accounted for this paradoxical conservation of V c through balancing negative osmotic effects resulting from the net cation efflux that would follow a titration of intracellular membrane‐impermeant anions by the intracellular accumulation of protons. It demonstrated that with established physiological values for intracellular buffering capacity and the permeability ratio of lactic acid and anionic lactate, P LacH : P Lac− , this would provide a mechanism that precisely balanced any effect on cell volume resulting from lactate accumulation during exercise.