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Control of interneurone firing pattern by axonal autoreceptors in the juvenile rat cerebellum
Author(s) -
MejiaGervacio Sheyla,
Marty Alain
Publication year - 2006
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2005.101675
Subject(s) - autoreceptor , neuroscience , afterdepolarization , bursting , postsynaptic potential , cerebellum , chemistry , electrophysiology , biology , agonist , receptor , biochemistry , repolarization
Recent work has shown that certain neurones have axonal GABA A receptors, whose tonic activation modifies their firing properties and neurotransmitter release capability. In addition, results obtained in interneurones of the molecular layer of the cerebellum indicate that action potential‐released GABA binds back to the axon that released it, generating an autoreceptor current. In the present paper, we show that at physiological Cl − i concentration (15 m m ) and at 34–36°C, the autoreceptor current generates a large amplitude (up to 21 mV) afterdepolarization that lasts for about 150 ms, and that occasionally leads to double firing. Furthermore we show that elimination of the afterdepolarization, by either blocking GABA A receptors, or eliminating the autoreceptor currents through prolonged whole‐cell recording, decreases burst firing. I h (a hyperpolarization‐activated current) was previously found to be prominent in interneurone axons. We show that blocking I h leads to an increase in the amplitude of the autoreceptor current as well as of the associated afterdepolarization, suggesting a shunting effect of I h on autoreceptor‐mediated afterdepolarization. Conversely, blocking I h accentuates burst firing. The effects of autoreceptor‐mediated afterdepolarization on firing are prominent during a period of development when interneurone synapses are stabilized and vanish by postnatal day 17 (PN 17), together with the expression of the autoreceptor current. Altogether, this work reveals a new role for autoreceptors in the regulation of cell excitability and firing pattern, which may contribute to the development and stabilization of the cerebellar network.