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Nitric oxide inhibition and the impact on renal nerve‐mediated antinatriuresis and antidiuresis in the anaesthetized rat
Author(s) -
Bagnall N. M.,
Dent P. C.,
Walkowska A.,
Sadowski J.,
Johns E. J.
Publication year - 2005
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2005.097709
Subject(s) - chemistry , nitric oxide , endocrinology , renal blood flow , medicine , stimulation , renal function , excretory system , diuresis , renal physiology , kidney , natriuresis , biochemistry
The contribution of nitric oxide (NO) to the antinatriuresis and antidiuresis caused by low‐level electrical stimulation of the renal sympathetic nerves (RNS) was investigated in rats anaesthetized with chloralose–urethane. Groups of rats, n = 6, were given i.v. infusions of vehicle, l ‐NAME (10 μg kg −1 min −1 ), 1400W (20 μg kg −1 min −1 ), or S ‐methyl‐thiocitrulline (SMTC) (20 μg kg −1 min −1 ) to inhibit NO synthesis non‐selectively or selectively to block the inducible or neuronal NOS isoforms (iNOS and nNOS, respectively). Following baseline measurements of blood pressure (BP), renal blood flow (RBF), glomerular filtration rate (GFR), urine flow ( UV ) and sodium excretion ( U Na V ), RNS was performed at 15 V, 2 ms duration with a frequency between 0.5 and 1.0 Hz. RNS did not cause measurable changes in BP, RBF or GFR in any of the groups. In untreated rats, RNS decreased UV and U Na V by 40–50% (both P < 0.01), but these excretory responses were prevented in l ‐NAME‐treated rats. In the presence of 1400W i.v. , RNS caused reversible reductions in both UV and U Na V of 40–50% (both P < 0.01), while in SMTC‐treated rats, RNS caused an inconsistent fall in UV , but a significant reduction ( P < 0.05) in U Na V of 21%. These data demonstrated that the renal nerve‐mediated antinatriuresis and antidiuresis was dependent on the presence of NO, generated in part by nNOS. The findings suggest that NO importantly modulates the neural control of fluid reabsorption; the control may be facilitatory at a presynaptic level but inhibitory on tubular reabsorptive processes.