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Zinc inhibits human ClC‐1 muscle chloride channel by interacting with its common gating mechanism
Author(s) -
Duffield Michael D.,
Rychkov Grigori Y.,
Bretag Allan H.,
Roberts Michael L.
Publication year - 2005
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2005.091777
Subject(s) - gating , block (permutation group theory) , biophysics , chemistry , mutation , chloride channel , cysteine , stereochemistry , biochemistry , biology , combinatorics , mathematics , gene , enzyme
Transition metals block the muscle Cl − channel ClC‐1, which belongs to a large family of double‐barreled Cl − channels and transporters. In the Torpedo Cl − channel ClC‐0, Zn 2 + block is closely related to the common gating mechanism that opens and closes both pores of the channel simultaneously, and the mutation C212S, which locks the common gate open, also eliminates the block. In ClC‐1, however, previous results suggested that Zn 2 + block is independent of gating, and that the cysteine residues involved in Zn 2 + binding are in different positions to those that confer Zn 2 + sensitivity on ClC‐0. In this work, we show that Zn 2 + block of ClC‐1 is faster at hyperpolarized potentials where the channel is more likely to be in the closed state. Mutation C277S, equivalent to C212S in ClC‐0, which locks the common gate in ClC‐1 open, virtually eliminates Zn 2 + block. A mutation, V321A, which reduces open probability of the common gate, facilitated Zn 2 + block. These results demonstrate that Zn 2 + block is state dependent, acting on the common gate. The extent of the block, however, is not a simple function of the open probability of the common gate. The Q 10 of ∼13 of the time course of Zn 2 + block, which is significantly higher than the Q 10 of common gating transitions in WT ClC‐1, suggests that Zn 2 + binds to a very high temperature‐dependent low‐probability closed substate of the common gate, which has not yet been characterized in this channel.