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Reduced glycine transporter type 1 expression leads to major changes in glutamatergic neurotransmission of CA1 hippocampal neurones in mice
Author(s) -
Martina Marzia,
B.Turcotte MarieEve,
Halman Samantha,
Tsai Guochuan,
Tiberi Mario,
Coyle Joseph T.,
Bergeron Richard
Publication year - 2005
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2004.080655
Subject(s) - glutamatergic , neurotransmission , hippocampal formation , glycine , neuroscience , transporter , chemistry , hippocampus , biology , biochemistry , glutamate receptor , amino acid , receptor , gene
To investigate the effects of persistent elevation of synaptic glycine at Schaffer collateral–CA1 synapses of the hippocampus, we studied the glutamatergic synaptic transmission in acute brain slices from mice with reduced expression of glycine transporter type 1 (GlyT1+/−) as compared to wild type (WT) littermates using whole‐cell patch‐clamp recordings of CA1 pyramidal cells. We observed faster decay kinetics, reduced ifenprodil sensitivity and increased zinc‐induced antagonism in N ‐methyl‐ d ‐aspartate receptor (NMDAR) currents of GlyT1+/− mice. Moreover, the ratio α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid receptor (AMPAR)/NMDAR was decreased in mutants compared to WT. Surprisingly, this change was associated with a reduction in the number of AMPARs expressed at the CA1 synapses in the mutants compared to WT. Overall, these findings highlight the importance of GlyT1 in regulating glutamatergic neurotransmission.