Premium
Arteriolar network architecture and vasomotor function with ageing in mouse gluteus maximus muscle
Author(s) -
Bearden Shawn E.,
Payne Geoffrey W.,
Chisty Alia,
Segal Steven S.
Publication year - 2004
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2004.068262
Subject(s) - vasomotor , vasodilation , ageing , phenylephrine , anatomy , skeletal muscle , microcirculation , medicine , chemistry , muscle architecture , vasoconstriction , endocrinology , blood pressure
Physical diminishes with ageing, but little is known of how the microvascular supply to skeletal muscle fibres is affected. To test the hypothesis that ageing alters blood flow control, we investigated network architecture and vasomotor responses of arterioles in the gluteus maximus muscle of young (2–3 months), adult (12–14 months) and old (18–20 months) C57BL6 male mice ( n = 83) (Young, Adult and Old, respectively). Microvascular casts revealed that the total number, length and surface area of arteriolar segments (diameter, 10–50 μm) were not significantly different across age‐groups. However, for arterioles with diameter of 30 μm, tortuosity and branch angles increased with age ( P < 0.05) . In anaesthetized mice, second‐order (2A) distributing arterioles had similar resting (17 ± 1 μm) and maximal (37 ± 1 μm) diameters and similar responsiveness to cumulative (10 −10 −10 −4 m ) superfusion of acetylcholine or phenylephrine. With superfusate oxygen level raised from 0 to 21%, 2A arteriolar constriction in Young (11 ± 1 μm) was greater ( P < 0.05) than Adult and Old (5 ± 1 μm). Observed 1 mm upstream from microiontophoresis of ACh (1 μA, 1 s), conducted vasodilatation was 10 ± 1 μm in Young, 17 ± 1 μm in Adult and 6 ± 1 μm in Old ( P < 0.05) . With muscle contractions (2, 4 and 8 Hz; 30 s) arteriolar diameter increased similarly across age‐groups (6 ± 1, 11 ± 1 and 18 ± 1 μm, respectively). Muscle mass and active tension were similar across age‐groups yet postcontraction vasodilatation recovered more rapidly in Old versus Adult and Young ( P < 0.05) . With arteriolar network architecture maintained during ageing, the impairment in conducted vasodilatation and attenuation of postcontraction vasodilatation may compromise exercise tolerance.