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Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine
Author(s) -
Goto Kazunori,
Matsuoka Satoshi,
Noma Akinori
Publication year - 2004
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2004.063875
Subject(s) - small intestine , chemistry , biophysics , neuroscience , microbiology and biotechnology , anatomy , biology , biochemistry
To explore the electrophysiological properties of the interstitial cells of Cajal (ICCs) and fibroblast‐like cells (FLCs), we developed a new preparation by treating the murine small intestine with collagenase. This thin muscle layer preparation contained at least two types of interstitial cells around the enteric nerve bundles, and the cluster of smooth muscle cells displayed a rhythmic contraction. We morphologically identified ICCs and FLCs and conducted patch clamp experiments on each type of cell. The c‐kit‐positive CD34‐negative ICCs showed spontaneous and rhythmic potential fluctuations, and a large transient inward current was evoked by depolarization under voltage clamp conditions. Once the inward current was triggered, it took a regenerative time course and lasted approximately 500 ms. The current was inactivated by continuous depolarization, and by removal of external Ca 2+ . The application of acetylcholine (ACh) prolonged the duration of spontaneous depolarization as well as the depolarization‐induced inward current. This inward current showed a reversal potential of around +3 mV and was considered to be due to non‐selective cation channels. The c‐kit‐negative CD34‐positive FLCs showed irregular or regular potential fluctuations, and spontaneous outward current was observed under voltage clamp conditions. This outward current showed a reversal potential of around −80 mV and might be classified as a potassium current. We failed to observe major time‐ and voltage‐dependent currents except the above two currents in the interstitial cells.