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AMPA‐receptor activation regulates the diffusion of a membrane marker in parallel with dendritic spine motility in the mouse hippocampus
Author(s) -
Richards D. A.,
De Paola V.,
Caroni P.,
Gähwiler B. H.,
McKinney R. A.
Publication year - 2004
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2004.062091
Subject(s) - dendritic spine , microbiology and biotechnology , ampa receptor , dendritic filopodia , motility , fluorescence recovery after photobleaching , actin remodeling of neurons , actin cytoskeleton , biophysics , excitatory postsynaptic potential , biology , cytoskeleton , glutamate receptor , chemistry , postsynaptic potential , receptor , neuroscience , hippocampal formation , membrane , biochemistry , cell
Dendritic spines are the site of most excitatory connections in the hippocampus. We have investigated the diffusibility of a membrane‐bound green fluorescent protein (mGFP) within the inner leaflet of the plasma membrane using Fluorescence Recovery After Photobleaching. In dendritic spines the diffusion of mGFP was significantly retarded relative to the dendritic shaft. In parallel, we have assessed the motility of dendritic spines, and found an inverse correlation between spine motility and the rate of diffusion of mGFP. We then tested the influence of glutamate receptor activation or blockade, and the involvement of the actin cytoskeleton (using latrunculin A) on spine motility and mGFP diffusion. These results show that glutamate receptors regulate the mobility of molecules in the inner leaflet of the plasma membrane through an action upon the actin cytoskeleton, suggesting a novel mechanism for the regulation of postsynaptic receptor density and composition.