Open AccessCa2+ imaging of mouse neocortical interneurone dendrites: Contribution of Ca2+-permeable AMPA and NMDA receptors to subthreshold Ca2+dynamics
Author(s) -
Jesse H. Goldberg,
Rafael Yuste,
Gábor Tamás
Publication year - 2003
Publication title -
journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2003.042598
Subject(s) - neuroscience , ampa receptor , calcium imaging , parvalbumin , subthreshold conduction , dendritic spine , nmda receptor , calcium , calretinin , glutamate receptor , chemistry , dendritic spike , synapse , interneuron , excitatory postsynaptic potential , biophysics , receptor , biology , hippocampal formation , physics , inhibitory postsynaptic potential , biochemistry , immunohistochemistry , organic chemistry , transistor , quantum mechanics , voltage , immunology
In this second study, we have combined two-photon calcium imaging with whole-cell recording and anatomic reconstructions to directly characterize synaptically evoked calcium signals in three types of mouse V1 supragranular interneurones: parvalbumin-positive fast spikers (FS), calretinin-positive irregular spikers (IS), and adapting cells (AD). We observed that subthreshold synaptic activation evoked calcium signals locally restricted to individual dendritic compartments. These signals were mediated by NMDA receptors (NMDARs) in AD and IS cells, whereas in FS cells, calcium-permeable AMPA receptors (CP-AMPARs) provided an additional and kinetically distinct influx. Furthermore, even a single, subthreshold synaptic activation evoked a larger dendritic calcium influx than backpropagating action potentials. Our results demonstrate that NMDARs dominate subthreshold calcium dynamics in interneurones and reveal the functional contribution of CP-AMPARs to a specific subclass of cortical interneurone. These data highlight different strategies in dendritic signal processing by distinct classes of interneurones.