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AMPA‐sst2 somatostatin receptor interaction in rat hypothalamus requires activation of nmda and/or metabotropic glutamate receptors and depends on intracellular calcium
Author(s) -
Peineau Stéphane,
Potier Brigitte,
Petit Florence,
Dournaud Pascal,
Epelbaum Jacques,
Gardette Robert
Publication year - 2003
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2002.025890
Subject(s) - metabotropic glutamate receptor , ampa receptor , metabotropic glutamate receptor 5 , metabotropic glutamate receptor 7 , long term depression , metabotropic receptor , metabotropic glutamate receptor 6 , chemistry , glutamate receptor , metabotropic glutamate receptor 1 , metabotropic glutamate receptor 2 , medicine , nmda receptor , metabotropic glutamate receptor 4 , neuroscience , endocrinology , somatostatin , receptor , biology , biochemistry
Modulation of glutamatergic transmission by neuropeptides is an essential aspect of neuronal network activity. Activation of the hypothalamic somatostatin sst2 receptor subtype by octreotide decreases AMPA glutamate responses, indicating a central link between a neurohormonal and neuromodulatory peptide and the main hypothalamic fast excitatory neurotransmitter. In mediobasal hypothalamic slices, sst2 activation inhibits the AMPA component of glutamatergic synaptic responses but is ineffective when AMPA currents are pharmacologically isolated. In mediobasal hypothalamic cultures, the decrease of AMPA currents induced by octreotide requires a concomitant activation of sst2 receptors with either NMDA and/or metabotropic glutamate receptors. This modulation depends on changes in intracellular calcium concentration induced by calcium flux through NMDA receptors or calcium release from intracellular stores following metabotropic glutamate receptor activation. These results highlight an unusual regulatory mechanism in which the simultaneous activation of at least three different types of receptor is necessary to allow somatostatin‐induced modulation of fast synaptic glutamatergic transmission in the hypothalamus.