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Conformational basis for the Li + ‐induced leak current in the rat γ‐aminobutyric acid (GABA) transporter‐1
Author(s) -
MacAulay Nanna,
Zeuthen Thomas,
Gether Ulrik
Publication year - 2002
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2002.022897
Subject(s) - chemistry , conductance , biophysics , molar concentration , reversal potential , xenopus , gating , leak , gaba transporter , substrate (aquarium) , transporter , biochemistry , patch clamp , receptor , biology , mathematics , organic chemistry , combinatorics , gene , ecology , environmental engineering , engineering
The rat γ‐aminobutyric acid transporter‐1 (GAT‐1) was expressed in Xenopus laevis oocytes and the substrate‐independent Li + ‐induced leak current was examined using two‐electrode voltage clamp. The leak current was not affected by the addition of GABA and was not due to H + permeation. The Li + ‐bound conformation of the protein displayed a lower passive water permeability than that of the Na + ‐ and choline (Ch + )‐bound conformations and the leak current did not saturate with increasing amounts of Li + in the test solution. The mechanism that gives rise to the leak current did not support active water transport in contrast to the mechanism responsible for GABA translocation (≈330 water molecules per charge). Altogether, these data support the distinct nature of the leak conductance in relation to the substrate translocation process. It was observed that the leak current was inhibited by low millimolar concentrations of Na + (the apparent affinity constant, K ′ 0.5 = 3 m m ). In addition, it was found that the GABA transport current was sustained at correspondingly low Na + concentrations if Li + was present instead of choline. This is consistent with a model in which Li + can bind and substitute for Na + at the putative ‘first’ apparently low‐affinity Na + binding site. In the absence of Na + , this allows a Li + ‐permeable channel to open at hyperpolarized potentials. Occupancy of the ‘second’ apparently high‐affinity Na + binding site by addition of low millimolar concentrations of Na + restrains the transporter from moving into a leak conductance mode as well as allowing maintenance of GABA‐elicited transport‐associated current.