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Dynamic imaging of free cytosolic ATP concentration during fuel sensing by rat hypothalamic neurones: evidence for ATP‐independent control of ATP‐sensitive K + channels
Author(s) -
Ainscow Edward K.,
Mirshamsi Shirin,
Tang Teresa,
Ashford Michael L. J.,
Rutter Guy A.
Publication year - 2002
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2002.022434
Subject(s) - extracellular , adenosine triphosphate , intracellular , oligomycin , cytosol , membrane potential , chemiosmosis , biology , glycolysis , intracellular ph , atp synthase , biochemistry , chemistry , biophysics , microbiology and biotechnology , medicine , metabolism , atpase , enzyme
Glucose‐responsive (GR) neurons from hypothalamic nuclei are implicated in the regulation of feeding and satiety. To determine the role of intracellular ATP in the closure of ATP‐sensitive K + (K ATP ) channels in these cells and associated glia, the cytosolic ATP concentration ([ATP] c ) was monitored in vivo using adenoviral‐driven expression of recombinant targeted luciferases and bioluminescence imaging. Arguing against a role for ATP in the closure of K ATP channels in GR neurons, glucose (3 or 15 m m ) caused no detectable increase in [ATP] c , monitored with cytosolic luciferase, and only a small decrease in the concentration of ATP immediately beneath the plasma membrane, monitored with a SNAP25‐luciferase fusion protein. In contrast to hypothalamic neurons, hypothalamic glia responded to glucose (3 and 15 m m ) with a significant increase in [ATP] c . Both neurons and glia from the cerebellum, a glucose‐unresponsive region of the brain, responded robustly to 3 or 15 m m glucose with increases in [ATP] c . Further implicating an ATP‐independent mechanism of K ATP channel closure in hypothalamic neurons, removal of extracellular glucose (10 m m ) suppressed the electrical activity of GR neurons in the presence of a fixed, high concentration (3 m m ) of intracellular ATP. Neurons from both brain regions responded to 5 m m lactate (but not pyruvate) with an oligomycin‐sensitive increase in [ATP] c . High levels of the plasma membrane lactate‐monocarboxylate transporter, MCT1, were found in both cell types, and exogenous lactate efficiently closed K ATP channels in GR neurons. These data suggest that (1) ATP‐independent intracellular signalling mechanisms lead to the stimulation of hypothalamic neurons by glucose, and (2) these effects may be potentiated in vivo by the release of lactate from neighbouring glial cells.