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Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor‐differentiated PC12 Cells
Author(s) -
Ng YuenKeng,
Lu Xinghua,
Levitan Edwin S.
Publication year - 2002
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2002.021733
Subject(s) - vesicle , microbiology and biotechnology , secretory vesicle , neuropeptide , neurosecretion , actin , cytoplasm , nerve growth factor , mobilization , chemistry , neuropeptide y receptor , biology , membrane , neuroscience , biochemistry , receptor , history , archaeology
It has been speculated that neurosecretion can be enhanced by increasing the motion, and hence, the availability of cytoplasmic secretory vesicles. However, facilitator‐induced physical mobilization of secretory vesicles has not been observed directly in living cells, and recent experimental results call this hypothesis into question. Here, high resolution green fluorescent protein (GFP)‐based measurements in nerve growth factor‐differentiated PC12 cells are used to test whether altering dense core vesicle (DCV) motion affects neuropeptide release. Experiments with mycalolide B and jasplakinolide demonstrate that neuropeptidergic DCV motion at the ends of processes is proportional to F‐actin. Furthermore, Ba 2+ increases DCV mobility without detectably modifying F‐actin. Finally, we show that altering DCV motion by changing F‐actin or stimulating with Ba 2+ proportionally changes sustained neuropeptide release. Therefore, increasing DCV mobility facilitates prolonged neuropeptide release.