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Perturbation of synaptic vesicle delivery during neurotransmitter release triggered independently of calcium influx
Author(s) -
Congar Patrice,
Trudeau LouisEric
Publication year - 2002
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2002.020222
Subject(s) - neurotransmitter , exocytosis , synaptic vesicle , neurotransmission , ionomycin , kiss and run fusion , synaptic vesicle recycling , vesicle , calcium , chemistry , endocytosis , bulk endocytosis , ionophore , microbiology and biotechnology , biophysics , stimulation , endocrinology , biology , medicine , biochemistry , receptor , membrane , central nervous system , secretion , organic chemistry
Although much evidence suggests that calcium (Ca 2+ ) usually triggers synaptic vesicle exocytosis and neurotransmitter release, the role of Ca 2+ in vesicle endocytosis and in the delivery of fusion‐competent vesicles (i.e. mobilisation and/or priming) in nerve terminals remains unclear. To address this issue, we have studied synaptic vesicle dynamics in cultured rat neurones under conditions where neurotransmitter release is triggered independently of Ca 2+ using the secretagogue Ruthenium Red (RR). Using a prolonged stimulation protocol, we find that RR causes a rapid increase in neurotransmitter release followed by a gradually decrementing response. In contrast, when release is triggered by moderate membrane depolarisation caused by saline containing 18 m m K + , release is sustained. These observations suggest that when release is triggered independently of a rise in Ca 2+ , endocytosis or vesicle mobilisation/priming are perturbed. Using FM2‐10, a fluorescent indicator of synaptic vesicle cycling, we find that neurotransmitter release triggered by RR is accompanied by both uptake and release of this dye, thereby suggesting that vesicle endocytosis is not blocked. To evaluate whether synaptic vesicle mobilisation/priming is perturbed in the absence of a rise in Ca 2+ , we compared the kinetics of FM2‐10 loss during prolonged stimulation. While 18 m m K + induced gradual and continuous dye loss, RR only induced substantial dye loss during the first minute of stimulation. In the presence of low concentrations of the Ca 2+ ionophore ionomycin, release caused by RR was prolonged. Taken together, these results provide evidence suggesting that, although a rise in intraterminal Ca 2+ is not required for endocytosis, it is essential for the continuous delivery of fusion‐competent vesicles and to maintain neurotransmitter release during prolonged stimulation.