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Enhancement of presynaptic calcium current by cysteine string protein
Author(s) -
Chen Shan,
Zheng Xu,
Schulze Karen L.,
Morris Terry,
Bellen Hugo,
Stanley Elis F.
Publication year - 2002
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.2001.013397
Subject(s) - biophysics , endogeny , chemistry , vesicle , cysteine , microbiology and biotechnology , synaptic vesicle , synapse , voltage dependent calcium channel , neuroscience , calcium , biology , biochemistry , organic chemistry , membrane , enzyme
The isolated chick ciliary neuron calyx synapse preparation was used to test cysteine string protein (CSP) action on presynaptic N‐type Ca 2+ channels. Endogenous CSP was localized primarily to secretory vesicle clusters in the presynaptic nerve terminal. Introduction of recombinant CSP into the voltage clamped terminal resulted in a prominent increase in Ca 2+ current amplitude. However, this increase could not be attributed to a change in Ca 2+ channel kinetics, voltage dependence, prepulse inactivation, or G protein inhibition but was attributed to the recruitment of dormant channels. Secretory vesicle associated endogenous CSP may play an important role in enhancing Ca 2+ channel activity at the transmitter release site.