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G protein‐dependent inhibition of L‐type Ca2+ currents by acetylcholine in mouse pancreatic B‐cells.
Author(s) -
Gilon P,
Yakel J,
Gromada J,
Zhu Y,
Henquin J C,
Rorsman P
Publication year - 1997
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1997.sp021911
Subject(s) - acetylcholine , chemistry , muscarinic acetylcholine receptor , pertussis toxin , biophysics , inhibitory postsynaptic potential , gtp' , voltage dependent calcium channel , patch clamp , reversal potential , nifedipine , g protein , endocrinology , medicine , calcium , receptor , biochemistry , biology , organic chemistry , enzyme
1. The effect of acetylcholine (ACh) on voltage‐dependent Ca2+ currents in mouse pancreatic B‐cells was studied using the whole‐cell configuration of the patch‐clamp technique. 2. ACh (0.25‐250 microM) reversibly and dose‐dependently inhibited the Ca2+ current elicited by depolarizations from ‐80 mV to +10 mV. Maximal inhibition was observed at concentrations > 25 microM where it amounted to approximately 35%. The effect was voltage independent and prevented by atropine (10 microM) suggesting that it was mediated by muscarinic receptors. 3. The inhibitory action of ACh on the Ca2+ current was abolished when the cytoplasmic solution contained GDP beta S (2 mM) and became irreversible when the non‐hydrolysable GTP analogue GTP gamma S (10 microM) was included in the pipette. This indicates the participation of G proteins in the inhibitory effect of ACh but pretreatment of the cells with either pertussis or cholera toxin failed to prevent the effect of ACh on the Ca2+ current. 4. ACh remained equally effective as an inhibitor of the whole‐cell Ca2+ current in the presence of the L‐type Ca2+ channel agonist (‐)‐Bay K 8644 and after partial inhibition of the current by nifedipine. Addition of omega‐agatoxin IVA, omega‐conotoxin GVIA or omega‐conotoxin MVIIC neither affected the peak Ca2+ current amplitude nor the extent of inhibition produced by ACh. These pharmacological properties indicate that ACh acts by inhibiting L‐type Ca2+ channels. 5. The inhibitory action of ACh on the B‐cell Ca2+ current was not secondary to elevation of [Ca2+]i and ACh remained equally effective as an inhibitor when Ba2+ was used as the charge carrier, when [Ca2+]i was buffered to low concentrations using EGTA and under experimental conditions preventing the mobilization of Ca2+ from intracellular stores. 6. These results suggest that ACh reduces the whole‐cell Ca2+ current in the B‐cell through a G protein‐regulated, voltage‐ and Ca(2+)‐independent inhibition of L‐type Ca2+ channels.