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Activity and expression of Na(+)‐K(+)‐ATPase in human placental cytotrophoblast cells in culture.
Author(s) -
Clarson L H,
Glazier J D,
Greenwood S L,
Jones C J,
Sides M K,
Sibley C P
Publication year - 1996
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1996.sp021804
Subject(s) - cytotrophoblast , ouabain , syncytiotrophoblast , cell culture , biology , messenger rna , microbiology and biotechnology , gene expression , atpase , endocrinology , medicine , chemistry , biochemistry , placenta , fetus , sodium , enzyme , gene , genetics , pregnancy , organic chemistry
1. To determine whether there is a change during differentiation, the activity and expression of Na(+)‐K(+)‐ATPase were studied in mononucleate cytotrophoblast cells (18 h culture) and syncytiotrophoblast‐like cells (66 h culture). A choriocarcinoma‐derived cell line (JAr) which, unlike the cytotrophoblast cells, divides in culture, was also studied for comparison. 2. Na(+)‐K(+)‐ATPase activity was assessed by measurement of ouabain‐sensitive 86Rb+ uptake. Na(+)‐K(+)‐ATPase expression was determined by (i) measurement of [3H]ouabain binding and (ii) Northern hybridization to measure expression of alpha‐1 and beta 1‐subunit mRNA. 3. There was no significant difference in either activity or expression of Na(+)‐K(+)‐ATPase during differentiation of cytotrophoblast cells. However, expression of alpha 1‐ and beta 1‐subunit mRNA was significantly lower in 66 vs. 18 h cultured cytotrophoblast cells. 4. Both Na(+)‐K(+)‐ATPase activity and [3H]ouabain binding was significantly greater in JAr cells than either cytotrophoblast cell groups, although expression of alpha 1‐ and beta 1‐subunit mRNA was the same as cytotrophoblast cells cultured for 18 h. 5. It is concluded that N(+)‐K(+)‐ATPase activity and protein expression does not change during differentiation of cytotrophoblast cells but that there are changes in expression at the transcriptional or post‐transcriptional level.