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A novel physiological function for platelet‐derived growth factor‐BB in rat dermis.
Author(s) -
Rodt S A,
Ahlén K,
Berg A,
Rubin K,
Reed R K
Publication year - 1996
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1996.sp021584
Subject(s) - platelet derived growth factor , beta (programming language) , alpha (finance) , chemistry , platelet derived growth factor receptor , integrin , endocrinology , growth factor , medicine , dermis , platelet , receptor , anatomy , surgery , construct validity , computer science , patient satisfaction , programming language
1. The present experiments describe a role for platelet‐derived growth factor‐BB and cellular adhesion receptors towards extracellular matrix molecules (beta 1‐integrins) in control of interstitial fluid pressure (Pif). 2. Pif was measured in rat skin with sharpened glass capillaries (3‐7 microns) connected to a servocontrolled counter‐pressure system. 3. The collagen and laminin‐binding alpha 2 beta 1‐integrin is involved in the control of Pif since subdermal injection (5 microliters) of monoclonal hamster anti‐rat alpha 2 beta 1‐integrin IgG (anti‐alpha 2 beta 1) resulted in increased negativity of Pif. Control Pif averaged ‐0.88 +/‐ 0.23 mmHg (+/‐ S.D.) and decreased to ‐2.50 +/‐ 0.35 mmHg (P < 0.05) and ‐3.88 +/‐ 1.45 mmHg (P < 0.05) at anti‐alpha 2 beta 1 concentrations of 0.56 and 1.12 mg ml‐1, respectively. 4. The effect of anti‐alpha 2 beta 1 was abolished when platelet‐derived growth factor‐BB (PDGF‐BB) (200 ng ml‐1) was injected together with anti‐alpha 2 beta 1. 5. The time‐ and dose‐responses of PDGF‐BB to counteract increased negativity of Pif were studied further using dextran anaphylaxis as an experimental model inducing increased negativity of Pif in skin. Control Pif averaged ‐0.33 +/‐ 0.43 mmHg and fell to ‐4.10 +/‐ 1.47 mmHg within 10 min after dextran (P < 0.01). Subsequent subdermal injection of PDGF‐BB at 200 ng ml‐1 normalized Pif in 10‐20 min which became ‐1.37 +/‐ 1.23 mmHg (P < 0.01 versus dextran, P > 0.05 versus control). PDGF‐BB had little or no effect at 50 ng ml‐1. PDGF‐AA and basic fibroblast growth factor had no effect on Pif. 6. The in vivo function reported for PDGF‐BB has not been described previously and provides further evidence for active participation of connective tissue cells in control of Pif by altering tension on extracellular matrix structures.