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Activation of L‐arginine transport (system y+) and nitric oxide synthase by elevated glucose and insulin in human endothelial cells.
Author(s) -
Sobrevia L,
Nadal A,
Yudilevich D L,
Mann G E
Publication year - 1996
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1996.sp021185
Subject(s) - medicine , endocrinology , arginine , insulin , nitric oxide , stimulation , umbilical vein , glucose transporter , citrulline , intracellular , chemistry , prostacyclin , l glucose , biology , biochemistry , amino acid , in vitro , islet
1. Modulation of L‐arginine transport (system y+) and release of nitric oxide (NO) and prostacyclin (PGI2) by elevated glucose and insulin were investigated in human cultured umbilical vein endothelial cells. 2. Elevated glucose induced a time‐ (6‐12 h) and concentration‐dependent stimulation of L‐arginine transport, which was reversible and associated with a 3‐fold increase in intracellular cGMP accumulation (index of NO synthesis) and 75% decrease in PGI2 production. 3. Elevated glucose had no effect on the initial transport rates for L‐serine, L‐citrulline, L‐leucine, L‐cystine or 2‐deoxyglucose. 4. Resting membrane potential was unaffected by elevated glucose whereas basal intracellular [Ca2+] increased from 65 +/‐ 5 nM to 136 +/‐ 16 nM. 5. Insulin induced a protein synthesis‐dependent stimulation of L‐arginine transport and increased NO and PGI2 production in cells exposed to 5 mM glucose. 6. In cells exposed to high glucose, insulin downregulated elevated rates of L‐arginine transport and cGMP accumulation but had no effect on the depressed PGI2 production. 7. Our findings suggest that insulin's normal stimulatory action on human endothelial cell vasodilator pathways may be impaired under conditions of sustained hyperglycaemia.