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Modulation of calcium‐activated non‐selective cation channel activity by nitric oxide in rat brown adipose tissue.
Author(s) -
Koivisto A,
Nedergaard J
Publication year - 1995
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1995.sp020790
Subject(s) - nitric oxide , dithiothreitol , chemistry , sodium nitroprusside , patch clamp , biophysics , calcium , blockade , inorganic chemistry , biochemistry , receptor , enzyme , organic chemistry , biology
1. Single‐channel calcium‐activated non‐selective cation currents from isolated rat brown fat cells were measured using the inside‐out patch configuration of the patch‐clamp technique. The existence of a possible modulatory effect of nitric oxide on the putative redox‐modulatory site located on the intracellular side of the non‐selective cation channel was investigated. 2. The nitric oxide‐releasing substances nitroglycerin, sodium nitroprusside, S‐nitrosocysteine and S‐nitroso‐N‐acetyl‐D,L‐penicillamine (all at 100 microM) were able to block channel activity almost completely. 3. In each case the blockade was persistent and could not be washed away. Dithiothreitol (DTT, 2 mM) was able to reverse the blockade to a large extent, whereas oxidized DTT (2 mM) was without effect. 4. It was concluded that nitric oxide can modulate non‐selective cation channel activity by oxidizing sulfhydryl groups and that this effect can be reversed by reduction.