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Calcium entry through a subpopulation of AMPA receptors desensitized neighbouring NMDA receptors in rat dorsal horn neurons.
Author(s) -
Kyrozis A,
Goldstein P A,
Heath M J,
MacDermott A B
Publication year - 1995
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1995.sp020736
Subject(s) - ampa receptor , nmda receptor , long term depression , silent synapse , glutamate receptor , postsynaptic potential , receptor , neuroscience , ion channel linked receptors , chemistry , excitatory postsynaptic potential , kainate receptor , biophysics , microbiology and biotechnology , biology , biochemistry
1. A Ca(2+)‐dependent interaction between non‐NMDA and NMDA receptors was studied in embryonic rat dorsal horn neurons grown in tissue culture using perforated‐patch recording. Specifically, non‐NMDA receptors were found to induce reversible inhibition of NMDA receptors in a manner dependent on the presence of extracellular Ca2+. 2. Non‐NMDA receptor‐induced inhibition of NMDA receptors was mediated by the elevation of intracellular Ca2+ concentration produced by Ca2+ entry through a subpopulation of alpha‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionate (AMPA) non‐NMDA receptors. Furthermore, Ca2+ entry through the AMPA channels alone is sufficient for desensitization of NMDA channels to occur. 3. Imaging of neuritic sites of Ca2+ revealed that Ca(2+)‐permeable AMPA channels are often co‐localized with NMDA channels on dorsal horn neurons, indicating that the Ca(2+)‐mediated interaction between receptors may occur within small dendritic domains. 4. The ability of Ca(2+)‐permeable AMPA channels to inhibit adjacent NMDA channels may contribute to the postsynaptic integration of excitatory input.