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Chloride and bicarbonate transport in chick embryonic red blood cells.
Author(s) -
Sieger U,
Brahm J,
Baumann R
Publication year - 1994
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1994.sp020201
Subject(s) - dids , bicarbonate , chloride , chemistry , efflux , red blood cell , biophysics , chromatography , analytical chemistry (journal) , biochemistry , membrane , biology , organic chemistry
1. Unidirectional efflux of 36Cl‐ and H14CO3‐ from erythrocytes of 4‐ to 16‐day‐old chick embryos was measured under steady‐state conditions at 37 degrees C and pH 7.7. The efflux rates were high, > 3 s‐1, and were, therefore, measured by means of the continuous flow tube method. 2. At day 4 of development the range of permeability coefficients for bicarbonate and chloride (PHCO3 and PCl was 1‐30 x 10(‐4) cm s‐1, with average values of respectively 10 x 10(‐4) and 8 x 10(‐4) cm s‐1. However, the results can be divided into two groups, one with PHCO3 and PCl above 12 x 10(‐4) cm s‐1, and one with values below 5 x 10(‐4) cm s‐1. The same range of values was also obtained for day 6 erythrocytes, but the overlap is more conspicuous. At day 16, PHCO3 and PCl were respectively 9 x 10(‐4) and 6 x 10(‐4) cm s‐1 (37 degrees C, pH 7.7). In adult chicken red blood cells PHCO3 and PCl were respectively 7 x 10(‐4) and 4 x 10(‐4) cm s‐1, and in human red blood cells the respective values were 5.6 x 10(‐4) and 4 x 10(‐4) cm s‐1. 3. Chloride self‐exchange, measured at 0 degrees C, was almost completely inhibited by addition of 1 mM 4,4'‐diisothiocyanostilbene‐2,2'‐disulphonate (DIDS) at both days 6 and 16 of embryonic development, supporting the finding that the embryonic chick erythrocytes also have a transmembrane anion exchanger similar to that of other red cells.(ABSTRACT TRUNCATED AT 250 WORDS)