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Effects of hypoxia on membrane potential and intracellular calcium in rat neonatal carotid body type I cells.
Author(s) -
Buckler K J,
VaughanJones R D
Publication year - 1994
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1994.sp020143
Subject(s) - membrane potential , depolarization , carotid body , biophysics , calcium , channel blocker , chemistry , glomus cell , electrophysiology , intracellular , egta , endocrinology , medicine , biochemistry , biology , organic chemistry
1. We have studied the effects of hypoxia on membrane potential and [Ca2+]i in enzymically isolated type I cells of the neonatal rat carotid body (the principal respiratory O2 chemosensor). Isolated cells were maintained in short term culture (3‐36 h) before use. [Ca2+]i was measured using the Ca(2+)‐sensitive fluoroprobe indo‐1. Indo‐1 was loaded into cells using the esterified form indo‐1 AM. Membrane potential was measured (and clamped) in single isolated type I cells using the perforated‐patch (amphotericin B) whole‐cell recording technique. 2. Graded reductions in PO2 from 160 Torr to 38, 19, 8, 5 and 0 Torr induced a graded rise of [Ca2+]i in both single and clumps of type I cells. 3. The rise of [Ca2+]i in response to anoxia was 98% inhibited by removal of external Ca2+ (+1 mM EGTA), indicating the probable involvement of Ca2+ influx from the external medium in mediating the anoxic [Ca2+]i response. 4. The L‐type Ca2+ channel antagonist nicardipine (10 microM) inhibited the anoxic [Ca2+]i response by 67%, and the non‐selective Ca2+ channel antagonist Ni2+ (2 mM) inhibited the response by 77%. 5. Under voltage recording conditions, anoxia induced a reversible membrane depolarization (or receptor potential) accompanied, in many cases, by trains of action potentials. These electrical events were coincident with a rapid rise of [Ca2+]i. When cells were voltage clamped close to their resting potential (‐40 to ‐60 mV), the [Ca2+]i response to anoxia was greatly reduced and its onset was much slower.(ABSTRACT TRUNCATED AT 250 WORDS)