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Sodium current‐induced calcium signals in isolated guinea‐pig ventricular myocytes.
Author(s) -
Lipp P,
Niggli E
Publication year - 1994
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1994.sp020035
Subject(s) - verapamil , biophysics , endoplasmic reticulum , ryanodine receptor , chemistry , calcium , myocyte , intracellular , extracellular , patch clamp , voltage clamp , guinea pig , membrane potential , endocrinology , biochemistry , biology , receptor , organic chemistry
1. Na+ current (INa)‐induced Ca2+ transients were studied in ventricular myocytes isolated from adult guinea‐pig hearts. The fluorescent Ca2+ indicator fluo‐3 or a mixture of fluo‐3 and fura‐red were used in conjunction with confocal microscopy to follow the intracellular Ca2+ concentration while membrane currents were measured simultaneously with the whole‐cell configuration of the patch‐clamp technique. 2. Ca2+ release from the sarcoplasmic reticulum (SR) could be triggered either by Ca2+ current (ICa) or Na+ current (INa). Analysis of INa‐induced Ca2+ signals at higher temporal resolution revealed a faster upstroke of these transients when compared with those triggered by ICa. 3. In the presence of 20 microM ryanodine to block SR Ca2+ release ICa elicited a verapamil‐sensitive Ca2+ transient with a slow upstroke. INa also induced a residual Ca2+ transient that was insensitive to 10 microM verapamil and characterized by a rapid upstroke. 4. The existence of a residual Ca2+ transient in the absence of SR Ca2+ release and L‐type ICa indicates that INa is indeed able to evoke an increase in [Ca2+]i without uncontrolled activation of Ca2+ channels. 5. Substitution of extracellular Na+ by Li+ suppressed INa‐induced Ca2+ transients, suggesting that the Ca2+ release and the residual Ca2+ transient can only be elicited by influx of Na+ and not by Li+. This result supports the notion that both the residual Ca2+ transient as well as the INa‐induced Ca2+ release are mediated by the Na(+)‐Ca2+ exchange.(ABSTRACT TRUNCATED AT 250 WORDS)