GABAB receptor modulation of Ca2+ currents in rat sensory neurones by the G protein G(0): antisense oligonucleotide studies.

1. Calcium channel currents (IBa) were recorded in cultured dorsal root ganglion neurones (DRGs), 24‐32 h after microinjection with 20‐mer phosphorothioate antisense oligonucleotides complementary either to a G alpha o or a G alpha i unique sequence, or with a nonsense sequence. 2. The ability of the GABAB agonist (‐)‐baclofen (50 microM) to inhibit IBa was examined. The maximum peak current was inhibited by 35.3 +/‐ 4.0% (n = 11) in control non‐injected cells, and by 38.1 +/‐ 2.6% (n = 11) and 34.8 +/‐ 4.2% (n = 5) in nonsense‐ and G alpha i oligonucleotide‐injected cells. Following G alpha o oligonucleotide injection, (‐)‐baclofen inhibited IBa by 21.0 +/‐ 3.2% (n = 19). 3. Confocal immunocytochemical localization of G alpha o showed prominent staining at the plasma membrane in control DRGs, and this was also present in G alpha i and nonsense oligonucleotide‐injected cells. The G alpha o staining at the plasma membrane was reduced by 76% in G alpha o oligonucleotide‐injected cells. In contrast, confocal immunocytochemical localization of G alpha i showed immunostaining in the membrane and cytoplasm of control, G alpha o‐ and nonsense‐injected DRGs, whereas this was depleted by 68% in G alpha i oligonucleotide‐injected cells. 4. These results indicate that the GABAB receptor couples to voltage‐sensitive calcium channels via the G protein G(o) and not Gi, and that antisense oligonucleotides can be used to deplete G proteins in DRGs.