Premium
Block of current through T‐type calcium channels by trivalent metal cations and nickel in neural rat and human cells.
Author(s) -
Mlinar B,
Enyeart J J
Publication year - 1993
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1993.sp019835
Subject(s) - chemistry , lanthanum , patch clamp , divalent , voltage dependent calcium channel , inorganic chemistry , calcium , biochemistry , receptor , organic chemistry
1. The effects of the trivalent cations yttrium (Y3+), lanthanum (La3+), cerium (Ce3+), neodymium (Nd3+), gadolinium (Gd3+), holmium (Ho3+), erbium (Er3+), ytterbium (Yb3+) and the divalent cation nickel (Ni2+) on the T‐type voltage gated calcium channel (VGCC) were characterized by the whole‐cell patch clamp technique using rat and human thyroid C cell lines. 2. All the metal cations (M3+) studied, blocked current through T‐type VGCC (IT) in a concentration‐dependent manner. Smaller trivalents were the best T‐channel antagonists and potency varied inversely with ionic radii for the larger M3+ ions. Estimation of half‐maximal blocking concentrations (IC50s) for IT carried by 10 mM Ca2+ resulted in the following potency sequence: Ho3+ (IC50 = 0.107 microM) approximately Y3+ (0.117) approximately Yb3+ (0.124) > or = Er3+ (0.153) > Gd3+ (0.267) > Nd3+ (0.429) > Ce3+ (0.728) > La3+ (1.015) >> Ni2+ (5.65). 3. Tail current measurements and conditioning protocols were used to study the influence of membrane voltage on the potency of these antagonists. Block of IT by Ni2+, Y3+, La3+ and the lanthanides was voltage independent in the range from ‐200 to +80 mV. In addition, the antagonists did not affect macroscopic inactivation and deactivation of T‐type VGCC. 4. Increasing the extracellular Ca2+ concentration reduced the potency of IT block by Ho3+, indicative of competitive antagonism between this blocker and the permeant ion for a binding site. 5. The results suggest that the mechanism of metal cation block of T‐type VGCC is occlusion of the channel pore by the antagonist binding to a Ca2+/M3+ binding site, located out of the membrane electric field. 6. Block of T‐type VGCC by Y3+, lanthanides and La3+ differ from the inhibition of high voltage‐activated VGCC block in several respects: smaller cations are more potent IT antagonists; block is voltage independent and the antagonists do not permeate T‐type channels. These differences suggest corresponding structural dissimilarities in the permeation pathways of low and high voltage‐activated Ca2+ channels.