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Endothelin and endothelium‐derived relaxing factor control of basal renovascular tone in hydronephrotic rat kidneys.
Author(s) -
Gulbins E,
Hoffend J,
Zou A P,
Dietrich M S,
Schlottmann K,
Cavarape A,
Steinhausen M
Publication year - 1993
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1993.sp019830
Subject(s) - vasoconstriction , medicine , endocrinology , efferent , vasodilation , endothelium derived relaxing factor , endothelin receptor , chemistry , basal (medicine) , macula densa , endothelin 1 , angiotensin ii , blood vessel , renin–angiotensin system , blood pressure , receptor , afferent , insulin
1. In order to investigate the control of renal vascular tone by endothelin (ET) and endothelium‐derived relaxing factor (EDRF) under basal conditions, we infused intravenously anti‐ET‐1/3 antibodies (a‐ET‐1/3) and NG‐nitro‐L‐arginine methyl ester (L‐NAME) in split hydronephrotic rat kidneys. 2. A 25 min I.V. infusion of a‐ET‐1/3 (4.0 x 10(‐13) mol kg‐1 min‐1) induced a time‐dependent vasodilatation of arcuate (16.5%) and interlobular arteries (18.6%) as well as an increase of glomerular blood flow (GBF) by 32%. 3. Inhibition of EDRF synthesis by L‐NAME produced a marked vasoconstriction of arcuate arteries (17.1%) and efferent (20.1%) arterioles and a decrease of GBF by 43%. 4. Co‐infusion of a‐ET‐1/3 and L‐NAME induced efferent vasoconstriction by 19.5%, whereas preglomerular vessel diameters remained unchanged. 5. The specificity of a‐ET‐1/3 effects was confirmed by simultaneous I.V. application of a‐ET‐1/3 and ET‐1 (160 ng I.V.) which produced no significant vascular effects. Injection of ET‐1 alone constricted arcuate arteries and decreased glomerular blood flow by 25%. 6. Experiments in normal rat kidneys with a‐ET‐1/3 I.V. revealed an increase of renal blood flow by 21%. 7. Our results demonstrate a physiological control of basal vascular tone in larger preglomerular arterioles by ET and EDRF. Efferent arteriolar tone is predominantly controlled by EDRF.