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Two types of neurone in the rat ventral tegmental area and their synaptic inputs.
Author(s) -
Johnson S W,
North R A
Publication year - 1992
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1992.sp019136
Subject(s) - ventral tegmental area , hyperpolarization (physics) , chemistry , gabaa receptor , neuroscience , excitatory postsynaptic potential , nmda receptor , depolarization , inhibitory postsynaptic potential , dopamine , biophysics , biology , receptor , dopaminergic , biochemistry , organic chemistry , nuclear magnetic resonance spectroscopy
1. Intracellular recordings were made from 241 ventral tegmental neurones in slices of rat midbrain. Seventy‐seven per cent of neurones were hyperpolarized by dopamine (principal cells); 16% were hyperpolarized by opioid peptides (secondary cells). 2. Most principal cells fired spontaneously (1‐3 Hz) with a threshold of ‐53 mV; most secondary cells did not fire spontaneously. Action potentials of principal cells were longer (0.9 ms) than those of secondary cells (0.5 ms). 3. Focal electrical stimulation within the ventral tegmental area evoked a biphasic synaptic potential, depolarization followed by hyperpolarization, with a duration of about 200 ms. Experiments with receptor antagonists showed that the depolarizing component resulted from activation of both N‐methyl‐D‐aspartate (NMDA) and non‐NMDA receptors and the hyperpolarizing component resulted from activation of GABAA receptors. 4. A later hyperpolarizing synaptic potential developed after a latency of 50 ms, reached its peak in 250 ms and had a duration of about 1 s. It reversed polarity at ‐108 mV (external potassium concentration was 2.5 mM), was blocked by phaclofen (30 microM‐1 mM) or 2‐hydroxysaclofen (100‐300 microM). In some cells, a phaclofen‐resistant component remained that was increased by cocaine and blocked by sulpiride (1 microM). 5. It is concluded that the ventral tegmental area contains two types of neurone having properties similar to those in the substantia nigra. The cells receive synaptic inputs mediated by excitatory amino acids acting at NMDA and non‐NMDA receptors, GABA acting at GABAA and GABAB receptors, and dopamine acting at D2 receptors.