Premium
Effects of proteins on the permeability of monolayers of cultured bovine arterial endothelium.
Author(s) -
Turner M R
Publication year - 1992
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1992.sp019072
Subject(s) - endothelium , permeability (electromagnetism) , chemistry , microbiology and biotechnology , medicine , biology , biochemistry , membrane
1. Monolayers of arterial endothelium on porous membranes were exposed to a pressure of 15 cmH2O at 37 degrees C, or of 30 cmH2O at 0 degree C. At constant pressure, the rate of liquid flow per unit area (Jv/A) through each monolayer decreased with time, in the way previously described for cultured endothelium. This phenomenon has been called sealing. After Jv/A stabilized, the pressure was reduced and the hydraulic permeability (Lp) of the endothelium was calculated from the relationship between Jv/A and pressure. Endothelium was seen to be damaged after some experiments at 37 degrees C, but appeared undamaged after experiments at 0 degree C. 2. Bovine serum albumin (BSA) did not influence the Lp of cultured endothelium. At 37 degrees C, the mean (+/‐ S.E.M.) endothelial Lp was 47.2 +/‐ 7.3 x 10(‐7) cm s‐1 cmH2O‐1 (n = 10) in the presence of BSA (5 g (100 ml)‐1). This is not significantly different from the mean (+/‐ S.E.M.) Lp of 53.4 +/‐ 9.0 x 10(‐7) cm s‐1 cmH2O‐1 (n = 9) in the absence of added protein (P greater than 0.10). At 0 degree C also, there was no significant difference between mean Lps in the presence of BSA (0.1 g (100 ml)‐1) and in the absence of added protein. 3. Solutions of BSA (5 g (100 ml)‐1 or of the neutral polymer Ficoll 70 (4 g (100 ml)‐1) did not exert any effective osmotic pressure across endothelium at 37 or 0 degrees C, respectively. 4. BSA (0.1 g (100 ml)‐1) did not enable solutions of Ficoll 70 (4 g (100 ml)‐1) to exert an effective osmotic pressure across endothelium at 0 degree C. 5. The mean Lp of endothelium at 0 degree C was significantly lower in the presence of cationized ferritin (CF; 0.1 g (100 ml)‐1) than in the absence of added protein (P less than 0.001). Native ferritin (NF; 0.1 g (100 ml)‐1) had no effect on Lp. 6. In the presence of CF (0.1 g (100 ml)‐1), solutions of Ficoll 70 (4 g (100 ml)‐1) exerted a mean effective osmotic pressure of 27.7 cmH2O (n = 5) across endothelium at 0 degree C. The mean effective osmotic pressure exerted across endothelium by solutions of Ficoll 70 (4 g (100 ml)‐1) plus NF (0.1 g (100 ml)‐1) was 1.2 cmH2O (n = 4).