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Tetraethylammonium blockade of apamin‐sensitive and insensitive Ca2(+)‐activated K+ channels in a pituitary cell line.
Author(s) -
Lang D G,
Ritchie A K
Publication year - 1990
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1990.sp018095
Subject(s) - apamin , tetraethylammonium , charybdotoxin , chemistry , repolarization , hyperpolarization (physics) , potassium channel , bk channel , biophysics , channel blocker , tetraethylammonium chloride , patch clamp , iberiotoxin , sk channel , potassium channel blocker , membrane potential , inward rectifier potassium ion channel , endocrinology , electrophysiology , medicine , ion channel , stereochemistry , biochemistry , potassium , biology , receptor , calcium , organic chemistry , nuclear magnetic resonance spectroscopy
1. The pharmacological sensitivities and physiological contributions of two types of Ca2(+)‐activated K+ channels (BK and SK) in GH3 cells were examined by the outside‐out, whole‐cell and cell‐attached modes of the patch‐clamp technique. 2. BK channels (250‐300 pS in symmetrical 150 mM‐K+) in outside‐out patches were blocked by external tetraethylammonium (TEA) and by 50 nM‐charybdotoxin (CTX), but were not blocked by apamin. 3. SK channels (9‐14 pS in symmetrical 150 mM‐K+) in outside‐out patches were blocked by external TEA and by apamin, but were not blocked by 50 nM‐CTX. 4. The dissociation constant (Kd) for TEA block of SK channels (3.1 +/‐ 0.37 mM) was 12‐fold greater than the Kd for the BK channels (260 +/‐ 21 microM). The TEA blockade of both channels was not strongly voltage dependent: for both channels the TEA binding site sensed less than 20% of the membrane electric field. 5. Application of blockers of the BK channels (1 mM‐TEA and 50 nM‐CTX) to whole cells under current clamp prolonged action potential duration; whereas application of apamin, a selective blocker of the SK channel, inhibited a slowly decaying after‐hyperpolarization and had little effect on action potential duration. Apamin also increased the firing rate in 30% of the spontaneously pacing cells. 6. It is suggested that BK channels contribute to action potential repolarization: whereas SK channels contribute to the regulation of action potential firing rate.