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Diabetes mellitus and sugar transport across the brush‐border and basolateral membranes of rat jejunal enterocytes.
Author(s) -
Debnam E S,
Ebrahim H Y,
Swaine D J
Publication year - 1990
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1990.sp018052
Subject(s) - brush border , enterocyte , diabetes mellitus , epithelial polarity , medicine , endocrinology , glucose transporter , galactose , in vivo , glucose uptake , epithelium , biology , streptozotocin , chemistry , cell , small intestine , membrane , biochemistry , insulin , vesicle , genetics , microbiology and biotechnology
1. The effects of streptozotocin‐induced diabetes mellitus on active jejunal glucose uptake in vivo, and on galactose movement across the brush‐border (phlorhizin‐sensitive) and basolateral (phlorhizin‐insensitive) membranes of isolated upper and mid‐villus enterocytes has been studied. 2. Chronic diabetes increased unidirectional phlorhizin‐sensitive galactose uptake by mid‐villus but not upper villus cells. In contrast, phlorhizin‐insensitive uptake by both cell populations was enhanced by diabetes. 3. Diabetes increased glucose absorption in vivo by mechanisms which were unrelated to hyperphagia. Mucosal hyperplasia acting together with an epithelium containing a higher proportion of mature enterocytes is the most likely explanation for the response. 4. We conclude that, during diabetes, the mid‐villus region is an important site of adaptation with functional changes occurring at both the brush‐border and basolateral membranes. The increased hexose transport ability of the basolateral membrane is retained during cell transit along the villus.