Premium
The effect of lactate on intracellular pH and force recovery of fatigued sartorius muscles of the frog, Rana pipiens.
Author(s) -
Renaud J M
Publication year - 1989
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1989.sp017747
Subject(s) - sartorius muscle , chemistry , tetanic stimulation , intracellular ph , lactic acid , stimulation , contraction (grammar) , intracellular , rana , biophysics , extracellular , anatomy , medicine , biochemistry , biology , genetics , receptor , neurotransmission , bacteria
1. The effects of pHo (extracellular pH) and lactic acid on pHi (intracellular pH) and tetanic force were examined in frog sartorius muscle. Ion‐selective microelectrodes were used to measure pHi. Tetanic force was elicited by field stimulation. Experiments were performed in HEPES‐buffered solution equilibrated with 100% O2. 2. Mean pHi values (+/‐ S.E.M.) of unfatigued frog sartorius muscles were 7.14 +/‐ 0.02 and 7.05 +/‐ 0.09 at pHo 7.2 and 6.4, respectively. 3. A stimulation at a rate of one 100 ms tetanic contraction per second for 3 min reduced pHi to 6.21 +/‐ 0.09 and 6.20 +/‐ 0.04 at pHo 7.2 and 6.4, respectively. Meanwhile at pHo 7.2, the tetanic force (defined as the maximum force developed during a tetanus) decreased by 82.9 +/‐ 2.6%, the maximum rate of relaxation decreased by 92.9 +/‐ 0.9%, and the rate constant of the relaxation decreased by 88.5 +/‐ 1.6%. At pHo 6.4, the decrease in tetanic force, maximum rate of relaxation and rate constant were 90.6 +/‐ 1.8%, 93.8 +/‐ 0.5 and 87.5 +/‐ 2.7%, respectively. 4. The maximum rates of recovery of pHi following fatigue were 0.068 +/‐ 0.05 and 0.025 +/‐ 0.05 pH units min‐1 at pHo 7.2 and 6.4, respectively. Recovery of normal tetanic force and relaxation rate was also slower at acidic pHo than at neutral pHo. 5. In the presence of 40 mmol l‐1 L‐lactic acid at pHo 7.2, the maximum rate of pHi recovery following fatigue was only 0.027 +/‐ 0.03 pH units min‐1 at pHo 7.2. The presence of lactic acid also reduced the recovery of the relaxation phase, but not the recovery of tetanic force. 6. It is suggested that pHi recovery is not a limiting factor for tetanic force recovery and that the extracellular H+ inhibits tetanic force recovery by acting at a site located on the outer surface of the sarcolemma. The recovery of the relaxation phase is believed to be pHi dependent.