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Active transport of lead by the calcium pump in human red cell ghosts.
Author(s) -
Simons T J
Publication year - 1988
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1988.sp017323
Subject(s) - vanadate , calcium , chemistry , biophysics , efflux , lead (geology) , calcium pump , red cell , biochemistry , biology , enzyme , atpase , medicine , paleontology , organic chemistry
1. Resealed human red cell ghosts containing lead buffers bring about a net transfer of lead from the cell interior to the outside. This transfer is ATP dependent. 2. The active transport of lead is characterized by a Vmax (maximum velocity) of 11 mmol/(l cells.h) and a KM (Michaelis constant) of 5 x 10(‐8) M for internal Pb2+, at pH 6.8 and 37 degrees C. 3. Lead efflux is antagonized by internal calcium, and is inhibited by vanadate with the same IC50 (inhibition constant) with which vanadate inhibits calcium pumping. 4. It is concluded that lead is transported by the calcium pump.