Premium
ATP and other adenine compounds increase mechanical activity and inositol trisphosphate production in rat heart.
Author(s) -
Legssyer A,
Poggioli J,
Renard D,
Vassort G
Publication year - 1988
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1988.sp017157
Subject(s) - adenosine , purinergic receptor , medicine , endocrinology , inositol , chemistry , adenosine triphosphate , adenosine diphosphate , inositol phosphate , phentolamine , prazosin , propranolol , adenosine monophosphate , antagonist , biology , biochemistry , receptor , platelet , platelet aggregation
1. The effects of adenosine 5'‐triphosphate (ATP) and other adenine compounds were examined on rat papillary and right ventricular muscles in the presence of 10 microM‐propranolol, 10 microM‐atropine and 0.1 microM‐prazosin or 10 microM‐phentolamine. 2. Adenosine, adenosine 5'‐monophosphate (AMP), adenosine 5'‐diphosphate (ADP), ATP and alpha,beta‐methylene ATP (APCPP) produced small positive inotropic effects, sometimes preceded by transient negative effects. 3. 8‐Phenyltheophylline (8‐PT), a P1‐purinoceptor antagonist antagonized the negative effects and increased the positive inotropy induced by ATP and adenosine. 4. In the presence of APCPP, a P2‐purinergic agonist, ATP had only negative inotropic effects. 5. Adenosine and ATP increased inositol 1, 4, 5‐ and inositol 1, 3, 4‐trisphosphate as well as inositol mono‐ and bisphosphate formation. Maximal effects were obtained at concentrations of 0.5 mM. 6. APCPP increased inositol phosphate formation while 8‐PT did not prevent the effects of adenosine and ATP. 7. It is suggested that P2‐purinoceptor activation induces both a positive inotropy and an increase in inositol‐lipid metabolism in rat ventricular muscles.