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Cl‐ ‐ and K+‐dependent inhibitory postsynaptic potentials evoked by interneurones of the rat lateral geniculate nucleus.
Author(s) -
Crunelli V,
Haby M,
JassikGerschenfeld D,
Leresche N,
Pirchio M
Publication year - 1988
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1988.sp017073
Subject(s) - bicuculline , reversal potential , hyperpolarization (physics) , inhibitory postsynaptic potential , excitatory postsynaptic potential , chemistry , biophysics , postsynaptic potential , depolarization , nipecotic acid , membrane potential , acetylcholine , electrophysiology , lateral geniculate nucleus , neuroscience , gabaa receptor , neurotransmitter , endocrinology , biology , biochemistry , patch clamp , stereochemistry , receptor , visual cortex , nuclear magnetic resonance spectroscopy
1. Hyperpolarizing potentials evoked by electrical stimulation of the optic tract were studied in projection cells of the rat dorsal lateral geniculate nucleus (LGN) in vitro. In the same cells the effects of gamma‐amino butyric acid (GABA), baclofen and acetylcholine (ACh) were also investigated. 2. In the majority of cells a short‐ (SHP) (34 ms) and a long‐lasting (LHP) (240 ms) hyperpolarizing potential could be recorded in the presence and in the absence of a preceding EPSP. They were blocked by tetrodotoxin (1 microM) and were more sensitive than the monosynaptic EPSP to a low‐Ca2+‐high‐Mg2+ solution. 3. The SHP was associated with a marked decrease (75%) in input resistance, was blocked by bicuculline (1‐100 microM) and its reversal potential (‐67 mV) was dependent on the extracellular Cl‐ concentration. 4. The LHP was associated with a smaller decrease (45%) in input resistance and its reversal potential (‐76 mV) was dependent on the extracellular K+ concentration. It was increased by bicuculline (100% at 50 microM) and nipecotic acid (30% at 10 microM), blocked by Ba2+ (1 mM), and unaffected by eserine (1‐10 microM), neostigmine (1‐10 microM) or by recording with EGTA‐filled electrodes. In the presence of bicuculline, a single LHP was able to evoke, as a rebound response, a low‐threshold Ca2+ spike that was, however, not followed by another LHP (or any other long‐lasting hyperpolarization). 5. Ionophoretic applications of GABA evoked in the same cell a Cl‐ ‐dependent hyperpolarization (reversal potential: ‐65 mV) and/or depolarization, both of which were associated with a marked decrease (91%) in input resistance and abolished by bicuculline. GABA was also able to evoke a bicuculline‐insensitive, K+‐dependent hyperpolarization that had a reversal potential of ‐75 mV and was associated with a smaller decrease (43%) in input resistance. 6. Baclofen, applied by ionophoresis, pressure ejection or in the perfusion medium (1‐100 microM), produced a hyperpolarization that had a reversal potential of ‐79 mV and was associated with a decrease (45%) in input resistance. 7. In the majority of cells (thirty‐seven out of forty) ACh evoked a slow depolarization and only in three cells a hyperpolarization which had a reversal potential of ‐80 mV.(ABSTRACT TRUNCATED AT 400 WORDS)