Premium
Transduction of aminergic and peptidergic signals in enteric neurones of the guinea‐pig.
Author(s) -
Palmer J M,
Wood J D,
Zafirov D H
Publication year - 1987
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1987.sp016578
Subject(s) - vasoactive intestinal peptide , bombesin , medicine , adenosine , endocrinology , forskolin , second messenger system , calcitonin gene related peptide , histamine , cholecystokinin , stimulation , cyclase , cyclic adenosine monophosphate , adenylate kinase , gastrin releasing peptide , neuropeptide , chemistry , biology , receptor
1. The biogenic amines 5‐hydroxytryptamine (5‐HT) and histamine, and the peptides bombesin, gastrin‐releasing peptide (GRP), vasoactive intestinal peptide (VIP), cholecystokinin (CCK), substance P and calcitonin gene‐related peptide (CGRP) each mimicked slow synaptic excitation (slow e.p.s.p.) when applied to myenteric neurones of the guinea‐pig small intestine. 2. Stimulation of the catalytic activity of adenylate cyclase by forskolin and intraneuronal elevation of cyclic 3',5'‐adenosine monophosphate (cyclic AMP) also mimicked the slow e.p.s.p. and the actions of the aminergic and peptidergic messengers. 3. Adenosine prevented stimulation of adenylate cyclase by forskolin and abolished the slow e.p.s.p.‐like actions of forskolin. 4. Exposure of the neurones to adenosine prior to or during application of bombesin, GRP, VIP, CCK or histamine blocked the actions of these substances. 5. Pre‐treatment with adenosine did not suppress the slow e.p.s.p.‐like actions of substance P, CGRP or 5‐HT. 6. The results suggest that signal transduction for bombesin, GRP, VIP, CCK and histamine involves stimulation of adenylate cyclase and second messenger function of cyclic AMP. Transduction mechanisms for 5‐HT, substance P and CGRP appear not to involve second messenger function of cyclic AMP.