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Endothelium‐derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate‐mediated effect.
Author(s) -
Collins P,
Griffith T M,
Henderson A H,
Lewis M J
Publication year - 1986
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1986.sp016336
Subject(s) - sodium nitroprusside , chemistry , calcium , endothelium derived relaxing factor , endocrinology , extracellular , acetylcholine , medicine , guanosine , cyclic guanosine monophosphate , vascular smooth muscle , biophysics , biochemistry , nitric oxide , biology , smooth muscle , organic chemistry
1. Measurement of tension and 45Ca influx and efflux were used to study the effects of endothelium‐derived relaxing factor (EDRF), sodium nitroprusside and 8‐bromo‐cyclic guanosine monophosphate (GMP) on contractile responses and calcium movements in aortic ring preparations of the rabbit. 2. EDRF activity, induced by stimulating endothelium‐containing rings with acetylcholine, was associated with relaxation of noradrenaline‐constricted rings and with a marked reduction of noradrenaline‐stimulated increase in calcium influx. Sodium nitroprusside and 8‐bromo‐cyclic GMP had a similar effect in de‐endothelialized preparations. 3. EDRF also inhibited noradrenaline‐stimulated calcium efflux. Sodium nitroprusside and 8‐bromo‐cyclic GMP had a similar effect in de‐endothelialized preparations, both in the presence and absence of extracellular calcium. 4. The vascular smooth muscle relaxant effect of EDRF and of nitrovasodilators may be effected by a cyclic GMP‐mediated reduction of cytosolic calcium, through both inhibition of calcium influx and reduction of intracellular calcium release.