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Release of neuropeptide Y in response to splanchnic nerve stimulation in the conscious calf.
Author(s) -
Allen J M,
Bircham P M,
Bloom S R,
Edwards A V
Publication year - 1984
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1984.sp015507
Subject(s) - medicine , endocrinology , splanchnic nerves , splanchnic , stimulation , neuropeptide , hexamethonium , neuropeptide y receptor , chemistry , radioimmunoassay , hemodynamics , receptor
Plasma concentrations of neuropeptide Y were determined by means of a specific radioimmunoassay in the conscious calf. Significantly higher concentrations were found in the adrenal venous effluent (102 +/‐ 34 pmol/l) compared to systemic arterial plasma (11 +/‐ 7 pmol/l). Stimulation of the splanchnic nerves at 40 Hz in bursts for 1 s at 10 s intervals resulted in a slow sustained rise in plasma neuropeptide Y in both adrenal (peak 1124 +/‐ 217 pmol/l and peripheral (peak 771 +/‐ 182 pmol/l) samples. Experiments performed in adrenalectomized calves produced a closely similar release of neuropeptide Y when the nerves were stimulated intermittently in the same pattern and under the same conditions. Stimulation of the splanchnic nerves at 4 Hz continuously resulted in a smaller response. Pre‐treatment with hexamethonium completely abolished the rise in plasma neuropeptide Y concentrations.