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Influences on the expression of acetylcholine receptors on rat nodose neurones in cell culture.
Author(s) -
Baccaglini P I,
Cooper E
Publication year - 1982
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1982.sp014123
Subject(s) - nodose ganglion , cholinergic , acetylcholine , receptor , biology , glutamate receptor , serotonin , cell culture , microbiology and biotechnology , neuroscience , medicine , endocrinology , 5 ht receptor , vagus nerve , stimulation , biochemistry , genetics
1. Nodose neurones dissociated from new‐born rats were grown in culture in the absence or presence of cells from neonatal skeletal muscle or heart. 2. In cultures devoid of non‐neuronal cells cholinergic interactions between the neurones were common. In the presence of non‐neuronal cells such interactions were rare. 3. A decrease in the proportion of neurones responsive to ACh was primarily responsible for the reduced incidence of synaptic interactions. Non‐neuronal cells influenced the expression of ACh receptors in developing nodose neurones in culture. 4. Most neurones appeared susceptible to the non‐neuronal influence during the first week in culture. 5. Many nodose ganglion neurones, whether grown in the presence or absence of non‐neuronal cells, were sensitive to gamma‐aminobutyric acid and serotonin but were insensitive to glutamate, glycine and L‐epinephrine.