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The reflexion coefficient as a measure of transepithelial permeability in the isolated rabbit pancreas.
Author(s) -
Bonting S L,
De Pont J J,
FleurenJakobs A M,
Jansen J W
Publication year - 1980
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1113/jphysiol.1980.sp013526
Subject(s) - electrolyte , chemistry , mannitol , sucrose , sorbitol , glycerol , chromatography , sodium , biochemistry , organic chemistry , electrode
1. The reflexion coefficients of a number of non‐electrolytes and electrolytes have been determined in the isolated rabbit pancreas. 2. The reflexion coefficients of the following non‐electrolytes were: urea, ‐0.02; glycerol, 0.06; erythritol, 0.11; sorbitol, 0.41; mannitol, 0.42; arabinose, 0.72; xylose, 0.74, assuming a value of 1.00 for sucrose. 3. These values are equal within the experimental error to values previously obtained with a tracer technique for the same preparation, but they are significantly lower than those reported by other investigators for the isolated perfused cat pancreas. 4. Addition of 100 mM‐sucrose to the bathing medium resulted in proportionally increased Na+ and K+ concentrations in the secreted fluid. The secreted fluid remained isotonic with the bathing medium under all circumstances. 5. Addition of 10(‐5) M‐carbachol to the bathing medium led to a reduction in the reflexion coefficient of sucrose from 1 to 0.85, but only when 25 mM‐sucrose was used. 6. The reflexion coefficients of electrolytes were: NaCl, 0.50; KCl, 0.51; NaHCO3, 0.52 and choline chloride, 1.02. 7. It is concluded that the isolated rabbit pancreas is highly permeable, both to electrolytes and to small non‐electrolytes, probably being more leaky than any other epithelium studied so far.